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Image Search Results
Journal: The Journal of Neuroscience
Article Title: Tocopherol Derivative TFA-12 Promotes Myelin Repair in Experimental Models of Multiple Sclerosis
doi: 10.1523/JNEUROSCI.0774-13.2013
Figure Lengend Snippet: TFA-12 impacts on oligodendroglial cells in EAE lesions. A, B, Immunolabeling for NG2+ OPCs in EAE lesions of vehicle-treated (A) and TFA12-treated (B) mouse spinal cords. C, Cell counts in EAE lesions reveal a significant reduction in the number of NG2+ cells (arrows), under TFA-12 conditions. D, E, CC1 immunolabeling (arrows) in spinal cord EAE lesions from vehicle- and TFA-12-treated mice. F, Quantification of CC1+ cells shows a 1.6-fold increase of the number CC1+ oligodendrocytes in TFA-12-treated groups. The dashed lines outline the border of EAE, as defined by CD45 immunolabeling on adjacent sections. **p < 0.01, Student's t test. Scale bar, 50 μm.
Article Snippet: For in vivo experiments, the following antibodies were used: rabbit anti-MBP (1:100; Millipore) and mouse anti-MOG (1:20, 8-18C5 hybridoma; gift from C. Linington, University of Aberdeen, Aberdeen, UK) for myelin staining; rat anti-CD45 (1:100; Invitrogen) for immune cells;
Techniques: Immunolabeling